ISSN 2458-7834
 

Original Article 


The effects of short-interval postconditioning in preventing muscle ischemia-reperfusion injury in rats

Heval Selman Ozkan, Ali Murat Sonel, Saime Irkoren, Huray Karaca.

Abstract
Background: Effectiveness of ischemic postconditioning to salvage tissues from ischemia-reperfusion damage has been shown in animal models but exact timing and duration of reperfusion/reocclusion cycles have not been studied in the literature. In this study we have evaluated the effects of short-interval postconditioning and tried to elucidate the most suitable reperfusion/reocclusion regime.
Methods: From forty rats 5 groups have been created. After dissection, the femoral artery has been clamped to produce ischemia which was maintained for 4 hours. Then, all extremities were reperfused. The first group was IR (ischemia + reperfusion) control group. In the other 4 study groups after ischemia postconditioning for 5 seconds (PC5), postconditioning for 10 seconds (PC10), postconditioning for 15 seconds (PC15), and postconditioning for 20 seconds (PC20) were applied. The animals were sacrificed, the gastrocnemius muscles were excised, and tissue analyses were utilized biochemically and macroscopically. Superoxide dismutase and nitric oxide (SOD and NO) level and muscle viability were evaluated.
Results: The muscle viability was found to be statistically increased in the postconditioning groups compared to the control group. In the postconditioning PC 15 group, NO, and muscle necrosis levels significantly decreased compared with the control group. In the PC10 and PC15 groups tissue NO levels were decreased but only between PC15 group and other groups this finding was statistically significant.
Conclusion: Reperfusion injury can be prevented by short-interval postconditioning and 15 seconds × 10 times reperfusion-reocclusion
cycles were found to be the most suitable approach.

Key words: Postconditioning, muscle, reperfusion injury, oxidative stress


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Heval Selman Ozkan
Articles by Ali Murat Sonel
Articles by Saime Irkoren
Articles by Huray Karaca
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Heval Selman Ozkan, Ali Murat Sonel, Saime Irkoren, Huray Karaca. The effects of short-interval postconditioning in preventing muscle ischemia-reperfusion injury in rats. Hand Microsurg. 2018; 7(1): 1-8. doi:10.5455/handmicrosurg.250784


Web Style

Heval Selman Ozkan, Ali Murat Sonel, Saime Irkoren, Huray Karaca. The effects of short-interval postconditioning in preventing muscle ischemia-reperfusion injury in rats. https://handmicrosurgeryjournal.com/?mno=250784 [Access: January 09, 2024]. doi:10.5455/handmicrosurg.250784


AMA (American Medical Association) Style

Heval Selman Ozkan, Ali Murat Sonel, Saime Irkoren, Huray Karaca. The effects of short-interval postconditioning in preventing muscle ischemia-reperfusion injury in rats. Hand Microsurg. 2018; 7(1): 1-8. doi:10.5455/handmicrosurg.250784



Vancouver/ICMJE Style

Heval Selman Ozkan, Ali Murat Sonel, Saime Irkoren, Huray Karaca. The effects of short-interval postconditioning in preventing muscle ischemia-reperfusion injury in rats. Hand Microsurg. (2018), [cited January 09, 2024]; 7(1): 1-8. doi:10.5455/handmicrosurg.250784



Harvard Style

Heval Selman Ozkan, Ali Murat Sonel, Saime Irkoren, Huray Karaca (2018) The effects of short-interval postconditioning in preventing muscle ischemia-reperfusion injury in rats. Hand Microsurg, 7 (1), 1-8. doi:10.5455/handmicrosurg.250784



Turabian Style

Heval Selman Ozkan, Ali Murat Sonel, Saime Irkoren, Huray Karaca. 2018. The effects of short-interval postconditioning in preventing muscle ischemia-reperfusion injury in rats. Hand and Microsurgery, 7 (1), 1-8. doi:10.5455/handmicrosurg.250784



Chicago Style

Heval Selman Ozkan, Ali Murat Sonel, Saime Irkoren, Huray Karaca. "The effects of short-interval postconditioning in preventing muscle ischemia-reperfusion injury in rats." Hand and Microsurgery 7 (2018), 1-8. doi:10.5455/handmicrosurg.250784



MLA (The Modern Language Association) Style

Heval Selman Ozkan, Ali Murat Sonel, Saime Irkoren, Huray Karaca. "The effects of short-interval postconditioning in preventing muscle ischemia-reperfusion injury in rats." Hand and Microsurgery 7.1 (2018), 1-8. Print. doi:10.5455/handmicrosurg.250784



APA (American Psychological Association) Style

Heval Selman Ozkan, Ali Murat Sonel, Saime Irkoren, Huray Karaca (2018) The effects of short-interval postconditioning in preventing muscle ischemia-reperfusion injury in rats. Hand and Microsurgery, 7 (1), 1-8. doi:10.5455/handmicrosurg.250784





Most Viewed Articles
Most Accessed Articles

  • Giant intramuscular lipoma of arm: A case report and review of the literature
    Muzaffer Durmus, Ahmet Demirhan Dal, Abdul Kerim Yapici, Sedat Avsar, Yalcin Bayram
    Hand Microsurg. 2014; 3(3): 87-90
    » Abstract » doi: 10.5455/handmicrosurg.173820

  • Cubital tunnel syndrome due to heterotrophic ossification caused by radial head fracture: A case report
    Seyitali Gumustas, Haci Bayram Tosun, Ismail Agir, Abuzer Uludag
    Hand Microsurg. 2014; 3(1): 24-28
    » Abstract » doi: 10.5455/handmicrosurg.1577

  • Munchausen’s syndrome or pure self-mutilation? A case of self-inflicted tendon injury
    Burak Kaya, Servet Elcin Alpat, Mehmet Sonmez, Cem Cerkez, Savas Serel
    Hand Microsurg. 2014; 3(3): 83-86
    » Abstract » doi: 10.5455/handmicrosurg.172607

  • Radial nerve grafting in high energy humeral fractures
    Yusuf Gürbüz, Tahir Sadık Sügün, Kemal Özaksar, Murat Kayalar, Tulgar Toros, İbrahim Kaplan
    Hand Microsurg. 2012; 1(2): 60-64
    » Abstract » doi: 10.2399/emd.12.58077

  • The utility of onion extract gel containing topical allantoin and heparin after surgical treatment of upper extremity burn scars
    Mehmet Ihsan Okur, Alpagan Mustafa Yildirim, Bilsev Ince
    Hand Microsurg. 2014; 3(3): 74-79
    » Abstract » doi: 10.5455/handmicrosurg.172436

  • Most Downloaded
    Top Downloaded Articles

  • Bone island and hand involvement – A short review
    Ganesh Singh Dharmshaktu, Binit Singh
    Hand Microsurg. 2018; 7(2): 93-97
    » Abstract » doi: 10.5455/handmicrosurg.258665

  • Lipofibromatous hamartoma of the digital nerve: A case report
    Emin Sir, Alper Aksoy
    Hand Microsurg. 2018; 7(1): 45-48
    » Abstract » doi: 10.5455/handmicrosurg.255406

  • Love sign’s love for glomus tumor
    Ankit Shukla, Varun Verma, Roshni Shukla, Rajesh Chaudhary, Saurab Sharma, Sajid Mohammad
    Hand Microsurg. 2018; 7(2): 105-108
    » Abstract » doi: 10.5455/handmicrosurg.279749

  • Synovial chondromatosis of the wrist
    Murat Kayalar, Abuzer Uludag, Basak Doganavsargil Yakut, Ahmet Savran
    Hand Microsurg. 2018; 7(2): 109-111
    » Abstract » doi: 10.5455/handmicrosurg.269392

  • The three bony point relationship of the elbow-Why is there still a lack of consensus?
    Supreeth Nekkanti, Dama Kondaiah Naidu, Jasmine Sebastin, Kamal Prasad, Mohan Yeshwanth, Archana Meka
    Hand Microsurg. 2018; 7(2): 79-82
    » Abstract » doi: 10.5455/handmicrosurg.291476

  • Most Cited Articles
    Most Cited Articles